Purification:Article Title: Isolation, Purification, and Characterization of the PR Oxidase from Penicillium roqueforti
Article Snippet: .. The purified PR oxidase showed a single band by SDS-PAGE (Fig. ). table ft1 table-wrap mode="anchored" t5 caption a7 Protein source Purification step Total amt of protein (mg) Total activity (U) Sp act (U/mg) Fold Yield (%) Crude extract None 2,399 74,955 31 1.0 100.0 Polyfiber concentrate Ultrafiltration 1,344 53,406 40 1.3 71.3 Precipitate Ammonium sulfate fractionation 580 37,073 64 2.1 49.5 Eluate Q-Sepharose column chromatography 261 23,393 90 2.9 31.2 Eluate Phenyl-TOYOPEARL 650 M column chromatography 104 18,424 177 5.7 24.6 Eluate Bio-Sil SEC-250 gel chromatography 38 7,638 201 6.5 10.2 Open in a separate window Purification of PR oxidase from P. roqueforti ATCC 48936 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 SDS-PAGE of the purified PR oxidase. ..
SDS Page:Article Title: Isolation, Purification, and Characterization of the PR Oxidase from Penicillium roqueforti
Article Snippet: .. The purified PR oxidase showed a single band by SDS-PAGE (Fig. ). table ft1 table-wrap mode="anchored" t5 caption a7 Protein source Purification step Total amt of protein (mg) Total activity (U) Sp act (U/mg) Fold Yield (%) Crude extract None 2,399 74,955 31 1.0 100.0 Polyfiber concentrate Ultrafiltration 1,344 53,406 40 1.3 71.3 Precipitate Ammonium sulfate fractionation 580 37,073 64 2.1 49.5 Eluate Q-Sepharose column chromatography 261 23,393 90 2.9 31.2 Eluate Phenyl-TOYOPEARL 650 M column chromatography 104 18,424 177 5.7 24.6 Eluate Bio-Sil SEC-250 gel chromatography 38 7,638 201 6.5 10.2 Open in a separate window Purification of PR oxidase from P. roqueforti ATCC 48936 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 SDS-PAGE of the purified PR oxidase. ..
Activity Assay:Article Title: Isolation, Purification, and Characterization of the PR Oxidase from Penicillium roqueforti
Article Snippet: .. The purified PR oxidase showed a single band by SDS-PAGE (Fig. ). table ft1 table-wrap mode="anchored" t5 caption a7 Protein source Purification step Total amt of protein (mg) Total activity (U) Sp act (U/mg) Fold Yield (%) Crude extract None 2,399 74,955 31 1.0 100.0 Polyfiber concentrate Ultrafiltration 1,344 53,406 40 1.3 71.3 Precipitate Ammonium sulfate fractionation 580 37,073 64 2.1 49.5 Eluate Q-Sepharose column chromatography 261 23,393 90 2.9 31.2 Eluate Phenyl-TOYOPEARL 650 M column chromatography 104 18,424 177 5.7 24.6 Eluate Bio-Sil SEC-250 gel chromatography 38 7,638 201 6.5 10.2 Open in a separate window Purification of PR oxidase from P. roqueforti ATCC 48936 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 SDS-PAGE of the purified PR oxidase. ..
Fractionation:Article Title: Isolation, Purification, and Characterization of the PR Oxidase from Penicillium roqueforti
Article Snippet: .. The purified PR oxidase showed a single band by SDS-PAGE (Fig. ). table ft1 table-wrap mode="anchored" t5 caption a7 Protein source Purification step Total amt of protein (mg) Total activity (U) Sp act (U/mg) Fold Yield (%) Crude extract None 2,399 74,955 31 1.0 100.0 Polyfiber concentrate Ultrafiltration 1,344 53,406 40 1.3 71.3 Precipitate Ammonium sulfate fractionation 580 37,073 64 2.1 49.5 Eluate Q-Sepharose column chromatography 261 23,393 90 2.9 31.2 Eluate Phenyl-TOYOPEARL 650 M column chromatography 104 18,424 177 5.7 24.6 Eluate Bio-Sil SEC-250 gel chromatography 38 7,638 201 6.5 10.2 Open in a separate window Purification of PR oxidase from P. roqueforti ATCC 48936 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 SDS-PAGE of the purified PR oxidase. ..
Column Chromatography:Article Title: Isolation, Purification, and Characterization of the PR Oxidase from Penicillium roqueforti
Article Snippet: .. The purified PR oxidase showed a single band by SDS-PAGE (Fig. ). table ft1 table-wrap mode="anchored" t5 caption a7 Protein source Purification step Total amt of protein (mg) Total activity (U) Sp act (U/mg) Fold Yield (%) Crude extract None 2,399 74,955 31 1.0 100.0 Polyfiber concentrate Ultrafiltration 1,344 53,406 40 1.3 71.3 Precipitate Ammonium sulfate fractionation 580 37,073 64 2.1 49.5 Eluate Q-Sepharose column chromatography 261 23,393 90 2.9 31.2 Eluate Phenyl-TOYOPEARL 650 M column chromatography 104 18,424 177 5.7 24.6 Eluate Bio-Sil SEC-250 gel chromatography 38 7,638 201 6.5 10.2 Open in a separate window Purification of PR oxidase from P. roqueforti ATCC 48936 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 SDS-PAGE of the purified PR oxidase. ..
Chromatography:Article Title: Isolation, Purification, and Characterization of the PR Oxidase from Penicillium roqueforti
Article Snippet: .. The purified PR oxidase showed a single band by SDS-PAGE (Fig. ). table ft1 table-wrap mode="anchored" t5 caption a7 Protein source Purification step Total amt of protein (mg) Total activity (U) Sp act (U/mg) Fold Yield (%) Crude extract None 2,399 74,955 31 1.0 100.0 Polyfiber concentrate Ultrafiltration 1,344 53,406 40 1.3 71.3 Precipitate Ammonium sulfate fractionation 580 37,073 64 2.1 49.5 Eluate Q-Sepharose column chromatography 261 23,393 90 2.9 31.2 Eluate Phenyl-TOYOPEARL 650 M column chromatography 104 18,424 177 5.7 24.6 Eluate Bio-Sil SEC-250 gel chromatography 38 7,638 201 6.5 10.2 Open in a separate window Purification of PR oxidase from P. roqueforti ATCC 48936 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 SDS-PAGE of the purified PR oxidase. ..
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